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Research
Interest:
I
am currently investigating the interaction of prolyl-tRNA synthetase
with the
free-standing editing domain, YbaK.
Specifically, my goal is to better understand
the ternary complex formed
by E. coli YbaK, E.
coli ProRS, and tRNAPro. To realize these goals, I
will perform
fluorescence-based binding assays with wild-type and truncation mutants
to
determine the importance of certain protein domains in binding. This
work is
based on previous studies by Songon An in the Musier-Forsyth lab (1).
References
(1)
Cys-tRNAPro editing by the Haemophilus
influenzae YbaK via
a novel synthetase/YbaK/tRNA ternary complex. Songon An
and Karin
Musier-Forsyth (2005) J. Biol. Chem. 280,
34465-72.
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